Screening for the Most Suitable Reference Genes for Gene Expression Studies in Equine Milk Somatic Cells.

ABSTRACT: Apart from the well-known role of somatic cell count as a parameter reflecting the inflammatory status of the mammary gland, the composition of cells isolated from milk is considered as a valuable material for gene expression studies in mammals. Due to its unique composition, in recent years an increasing interest in mare's milk consumption has been observed. Thus, investigating the genetic background of horse's milk variability presents and interesting study model. Relying on 39 milk samples collected from mares representing three breeds (Polish Primitive Horse, Polish Cold-blooded Horse, Polish Warmblood Horse) we aimed to investigate the utility of equine milk somatic cells as a source of mRNA and to screen the best reference genes for RT-qPCR using geNorm and NormFinder algorithms. The

SUBMITTER: Cieslak J 

PROVIDER: S-EPMC4593561 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature