Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis.

ABSTRACT: Studying the complex relationship between transcription, translation and protein degradation is essential to our understanding of biological processes in health and disease. The limited correlations observed between mRNA and protein abundance suggest pervasive regulation of post-transcriptional steps and support the importance of profiling mRNA levels in parallel to protein synthesis and degradation rates. In this work, we applied an integrative multi-omic approach to study gene expression along the mammalian cell cycle through side-by-side analysis of mRNA, translation and protein levels. Our analysis sheds new light on the significant contribution of both protein synthesis and degradation to the variance in protein expression. Furthermore, we find that translation regulation plays an imp

SUBMITTER: Aviner R 

PROVIDER: S-EPMC4595013 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature