Pathogen-regulated genes in wheat isogenic lines differing in resistance to brown rust Puccinia triticina.

ABSTRACT:

Background

Inoculation of wheat plants with Puccinia triticina (Pt) spores activates a wide range of host responses. Compatible Pt interaction with susceptible Thatcher plants supports all stages of the pathogen life cycle. Incompatible interaction with TcLr9 activates defense responses including oxidative burst and micronecrotic reactions associated with the pathogen's infection structures and leads to complete termination of pathogen development. These two contrasting host-pathogen interactions were a foundation for transcriptome analysis of incompatible wheat-Pt interaction.

Methods

A suppression subtractive hybridization (SSH) library was constructed using cDNA from pathogen-inoculated susceptible Thatcher and resistant TcLr9 isogenic lines. cDNA represented steps of whe

SUBMITTER: Dmochowska-Boguta M 

PROVIDER: S-EPMC4595183 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature