Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species.

ABSTRACT:

Background

Sex determination in mammals requires expression of the Y-linked gene Sry in the bipotential genital ridges of the XY embryo. Even minor delay of the onset of Sry expression can result in XY sex reversal, highlighting the need for accurate gene regulation during sex determination. However, the location of critical regulatory elements remains unknown. Here, we analysed Sry flanking sequences across many species, using newly available genome sequences and computational tools, to better understand Sry's genomic context and to identify conserved regions predictive of functional roles.

Methods

Flanking sequences from 17 species were analysed using both global and local sequence alignment methods. Multiple motif searches were employed to characterise common motifs in ot

SUBMITTER: Larney C 

PROVIDER: S-EPMC4595323 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature