Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary, and Type 2 Diabetic Males.

ABSTRACT:

Context

The role of perilipin 3 (PLIN3) on lipid oxidation is not fully understood.

Objective

We aimed to 1) determine whether skeletal muscle PLIN3 protein content is associated with lipid oxidation in humans, 2) understand the role of PLIN3 in lipid oxidation by knocking down PLIN3 protein content in primary human myotubes, and 3) compare PLIN3 content and its role in lipid oxidation in human primary skeletal muscle cultures established from sedentary, healthy lean (leans), type 2 diabetic (T2D), and physically active donors.

Design, participants, and intervention

This was a clinical investigation of 29 healthy, normoglycemic males and a cross-sectional study using primary human myotubes from five leans, four T2D, and four active donors. Energy expenditure, whole-bo

SUBMITTER: Covington JD 

PROVIDER: S-EPMC4596049 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature