In silico prediction of Ebola Zaire GP(1,2) immuno-dominant epitopes for the Balb/c mouse.

ABSTRACT:

Background

Ebola is a Filovirus (FV) that induces a highly communicable and deadly hemorrhagic fever. Currently, there are no approved vaccines to treat FV infections. Protection from FV infection requires cell mediated and humoral immunity. Glycoprotein GP(1,2) Fc Zaire, a recombinant FV human Fc fusion protein, has been shown to confer protection against mouse adapted Zaire Ebola virus. The present studies are focused upon identifying immunodominant epitopes using in silico methods and developing tetramers as a diagnostic reagent to detect cell mediated immune responses to GP(1,2) Fc.

Methods

The GP(1,2) Ebola Zaire sequence from the 1976 outbreak was analyzed by both BIMAS and SYFPEITHI algorithms to identify potential immuno-dominant epitopes. Several peptides were synth

SUBMITTER: Dutta DK 

PROVIDER: S-EPMC4596288 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature