BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis.

ABSTRACT: Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol-cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol-cytochrome c reductase iron-sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, an

SUBMITTER: Liu T 

PROVIDER: S-EPMC4596333 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature