Multiplex Assay for Protein Profiling and Potency Measurement of German Cockroach Allergen Extracts.

ABSTRACT:

Background

German cockroach (GCr) allergens induce IgE responses and may cause asthma. Commercial GCr allergen extracts are variable and existing assays may not be appropriate for determining extract composition and potency.

Objective

Our aim was to develop a multiplex antibody/bead-based assay for assessment of GCr allergen extracts.

Methods

Single chain fragment variable (scFv) antibodies against GCr were obtained by screening libraries derived from naïve human lymphocytes and hyperimmunized chicken splenocytes and bone marrow. Selected clones were sequenced and characterized by immunoblotting. Eighteen scFv antibodies (17 chicken, 1 human) coupled to polystyrene beads were used in this suspension assay; binding of targeted GCr allergens to antibody-coated beads was

SUBMITTER: Khurana T 

PROVIDER: S-EPMC4596881 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature