Morphine Regulated Synaptic Networks Revealed by Integrated Proteomics and Network Analysis.

ABSTRACT: Despite its efficacy, the use of morphine for the treatment of chronic pain remains limited because of the rapid development of tolerance, dependence and ultimately addiction. These undesired effects are thought to be because of alterations in synaptic transmission and neuroplasticity within the reward circuitry including the striatum. In this study we used subcellular fractionation and quantitative proteomics combined with computational approaches to investigate the morphine-induced protein profile changes at the striatal postsynaptic density. Over 2,600 proteins were identified by mass spectrometry analysis of subcellular fractions enriched in postsynaptic density associated proteins from saline or morphine-treated striata. Among these, the levels of 34 proteins were differentially alter

SUBMITTER: Stockton SD 

PROVIDER: S-EPMC4597137 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature