An automated Genomes-to-Natural Products platform (GNP) for the discovery of modular natural products.

ABSTRACT: Bacterial natural products are a diverse and valuable group of small molecules, and genome sequencing indicates that the vast majority remain undiscovered. The prediction of natural product structures from biosynthetic assembly lines can facilitate their discovery, but highly automated, accurate, and integrated systems are required to mine the broad spectrum of sequenced bacterial genomes. Here we present a genome-guided natural products discovery tool to automatically predict, combinatorialize and identify polyketides and nonribosomal peptides from biosynthetic assembly lines using LC-MS/MS data of crude extracts in a high-throughput manner. We detail the directed identification and isolation of six genetically predicted polyketides and nonribosomal peptides using our Genome-to-Natural Pr

SUBMITTER: Johnston CW 

PROVIDER: S-EPMC4598715 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature