Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture.

ABSTRACT: To more closely mimic the native cellular microenvironment, 3D scaffolds derived from the extracellular matrix (ECM) are being developed as alternatives to conventional 2D culture systems. In the present study, we established methods to fabricate nonchemically cross-linked 3D porous foams derived entirely from decellularized porcine left ventricle (DLV) for use as an in vitro cardiac cell culture platform. Furthermore, we explored the effects of physically preprocessing the DLV through mechanical mincing versus cryomilling, as well as varying the ECM concentration on the structure, composition, and physical properties of the foams. Our results indicate that the less highly processed minced foams had a more cohesive and complex network of ECM components, enhanced mechanical properties, and

SUBMITTER: Russo V 

PROVIDER: S-EPMC4598938 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature