Hypoxic niche-mediated regeneration of hematopoiesis in the engraftment window is dominantly affected by oxygen tension in the milieu.

ABSTRACT: The bone marrow (BM) microenvironment or the hematopoietic stem cell (HSC) niche is normally hypoxic, which maintains HSC quiescence. Paradoxically, transplanted HSCs rapidly proliferate in this niche. Pretransplant myelosuppression results in a substantial rise in oxygen levels in the marrow microenvironment due to reduced cellularity and consequent low oxygen consumption. Therefore, it may be construed that the rapid proliferation of the engrafted HSCs in the BM niche is facilitated by the transiently elevated oxygen tension in this milieu during the "engraftment window." To determine whether oxygen tension dominantly affects the regeneration of hematopoiesis in the BM niche, we created an "oxygen-independent hypoxic niche" by treating BM-derived mesenchymal stromal cells (BMSCs) with a

SUBMITTER: Moirangthem RD 

PROVIDER: S-EPMC4599134 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature