Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells.

ABSTRACT:

Introduction

There is a clinical need for developing systemic transplantation protocols for use of human skeletal stem cells (also known bone marrow stromal stem cells) (hBMSC) in tissue regeneration. In systemic transplantation studies, only a limited number of hBMSC home to injured tissues suggesting that only a subpopulation of hBMSC possesses "homing" capacity. Thus, we tested the hypothesis that a subpopulation of hBMSC defined by ability to form heterotopic bone in vivo, is capable of homing to injured bone.

Methods

We tested ex vivo and in vivo homing capacity of a number of clonal cell populations derived from telomerized hBMSC (hBMSC-TERT) with variable ability to form heterotopic bone when implanted subcutaneously in immune deficient mice. In vitro transwell migrat

SUBMITTER: Andersen RK 

PROVIDER: S-EPMC4599318 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature