Fluorescent filter-trap assay for amyloid fibril formation kinetics in complex solutions.

ABSTRACT: Amyloid fibrils are the most distinct components of the plaques associated with various neurodegenerative diseases. Kinetic studies of amyloid fibril formation shed light on the microscopic mechanisms that underlie this process as well as the contributions of internal and external factors to the interplay between different mechanistic steps. Thioflavin T is a widely used noncovalent fluorescent probe for monitoring amyloid fibril formation; however, it may suffer from limitations due to the unspecific interactions between the dye and the additives. Here, we present the results of a filter-trap assay combined with the detection of fluorescently labeled amyloid β (Aβ) peptide. The filter-trap assay separates formed aggregates based on size, and the fluorescent label attached to Aβ allows for

SUBMITTER: Nasir I 

PROVIDER: S-EPMC4599436 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature