Circulating microRNA Profiling Needs Further Refinement Before Clinical Use in Patients With Aortic Stenosis.

ABSTRACT:

Background

Aortic stenosis (AS) is a progressive condition leading to heart failure and death without treatment. No medical therapy currently exists for AS, and a major management challenge is deciding on the correct timing of aortic valve replacement. MicroRNAs (miRNAs) are short noncoding RNAs that are stable in the circulation. We wished to use miRNAs as biomarkers of disease in AS.

Methods and results

We performed microarray-based whole miRNome profiling of 24 participants with AS and 27 control participants. After adjustment for age and multiple testing, we identified 4 miRNAs significantly different between groups. These findings were then examined using quantitative polymerase chain reaction in a larger validation cohort of 101 controls and 94 participants with AS, st

SUBMITTER: Coffey S 

PROVIDER: S-EPMC4599470 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature