Genome expression profiling and network analysis of nitrite therapy during chronic ischemia: possible mechanisms and interesting molecules.

ABSTRACT: Sodium nitrite is widely recognized to be a highly effective NO donor for the treatment of several ischemic tissue disorders. However, mechanisms by which nitrite confers cytoprotection during ischemic disorders remain largely unknown. In this study, we used genome expression profiling approaches to evaluate changes in gene expression in the hind-limb ischemia model using vehicle or sodium nitrite therapy. Sodium nitrite significantly restored ischemic tissue perfusion by day 3 post-ligation which returned to normal by day 7. Genesifter analysis of Affymetrix GeneChip data revealed a significant down-regulation of gene expression profiles at day 3, whereas gene expression profiles were predominantly up-regulated at day 7. Ingenuity network analysis of gene expression profiles at day 3 show

SUBMITTER: Pattillo CB 

PROVIDER: S-EPMC4599788 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature