Relationships between putative G-quadruplex-forming sequences, RecQ helicases, and transcription.

ABSTRACT:

Background

Putative G-quadruplex-forming sequences (PQS) have long been implicated in regulation of transcription, though the actual mechanisms are not well understood. One proposed mechanism involves the activity of PQS-specific helicases belonging to the RecQ helicase family. However, patterns of PQS that correlate with transcriptional sensitivity to RecQ helicases are not well studied, and no adequate transcriptional model exists to account for PQS effects.

Methods

To better understand PQS transcriptional effects, we analyze PQS motifs in genes differentially-transcribed in Bloom Syndrome (BS) and Werner Syndrome (WS), two disorders resulting in loss of PQS-interacting RecQ helicases.  We also correlate PQS genome-wide with transcription in multiple human cells lines whil

SUBMITTER: Smestad JA 

PROVIDER: S-EPMC4599794 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature