Isolation and Characterization of Lytic Properties of Bacteriophages Specific for M. haemolytica Strains.

ABSTRACT:

Aim of study

The objective of this study was isolation and morphological characterization of temperate bacteriophages obtained from M. haemolytica strains and evaluation of their lytic properties in vitro against M. haemolytica isolated from the respiratory tract of calves.

Material and methods

The material for the study consisted of the reference strain M. haemolytica serotype 1 (ATCC®) BAA-410™, reference serotypes A1, A2, A5, A6, A7, A9 and A11, and wild-type isolates of M. haemolytica. Bacteriophages were induced from an overnight bacterial starter culture of all examined M. haemolytica strains treated with mitomycin C. The lytic properties and host ranges were determined by plaque assays. The morphology of the bacteriophages was examined in negative-stained smears with

SUBMITTER: Urban-Chmiel R 

PROVIDER: S-EPMC4599942 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature