Two Essential Light Chains Regulate the MyoA Lever Arm To Promote Toxoplasma Gliding Motility.

ABSTRACT:

Unlabelled

Key to the virulence of apicomplexan parasites is their ability to move through tissue and to invade and egress from host cells. Apicomplexan motility requires the activity of the glideosome, a multicomponent molecular motor composed of a type XIV myosin, MyoA. Here we identify a novel glideosome component, essential light chain 2 (ELC2), and functionally characterize the two essential light chains (ELC1 and ELC2) of MyoA in Toxoplasma. We show that these proteins are functionally redundant but are important for invasion, egress, and motility. Molecular simulations of the MyoA lever arm identify a role for Ca(2+) in promoting intermolecular contacts between the ELCs and the adjacent MLC1 light chain to stabilize this domain. Using point mutations predicted to ablate eith

SUBMITTER: Williams MJ 

PROVIDER: S-EPMC4600101 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature