Effect of Serotype on Pneumococcal Competition in a Mouse Colonization Model.

ABSTRACT:

Unlabelled

Competitive interactions between Streptococcus pneumoniae strains during host colonization could influence the serotype distribution in nasopharyngeal carriage and pneumococcal disease. We evaluated the competitive fitness of strains of serotypes 6B, 14, 19A, 19F, 23F, and 35B in a mouse model of multiserotype carriage. Isogenic variants were constructed using clinical strains as the capsule gene donors. Animals were intranasally inoculated with a mixture of up to six pneumococcal strains of different serotypes, with separate experiments involving either clinical isolates or isogenic capsule-switch variants of clinical strain TIGR4. Upper-respiratory-tract samples were repeatedly collected from animals in order to monitor changes in the serotype ratios using quantitative

SUBMITTER: Trzcinski K 

PROVIDER: S-EPMC4600102 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature