C-Type Cytochrome Assembly Is a Key Target of Copper Toxicity within the Bacterial Periplasm.

ABSTRACT:

Unlabelled

In the absence of a tight control of copper entrance into cells, bacteria have evolved different systems to control copper concentration within the cytoplasm and the periplasm. Central to these systems, the Cu(+) ATPase CopA plays a major role in copper tolerance and translocates copper from the cytoplasm to the periplasm. The fate of copper in the periplasm varies among species. Copper can be sequestered, oxidized, or released outside the cells. Here we describe the identification of CopI, a periplasmic protein present in many proteobacteria, and show its requirement for copper tolerance in Rubrivivax gelatinosus. The ΔcopI mutant is more susceptible to copper than the Cu(+) ATPase copA mutant. CopI is induced by copper, localized in the periplasm and could bind copper.

SUBMITTER: Durand A 

PROVIDER: S-EPMC4600104 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature