Cloning and Expression of Beta Subunit Gene of Phycocyanin From Spirulina platensis in Escherichia coli.

ABSTRACT:

Background

C-Phycocyanin (C-PC) from blue-green algae such as Spirulina has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-tumor activities. Recombinant β-subunit of C-PC (C-PC/β) is an inhibitor of cell proliferation and an inducer of cancer cell apoptosis.

Objectives

Since C-PC/β has a big potential to be used as a promising cancer prevention or therapy agent, the purpose of this study was to clone and express Spirulina platensis cpcB gene in a bacterial expression system. This is a significant step for the production of this compound.

Materials and methods

The cpcB gene was amplified using specific primers and cloned in a bacterial expression vector, namely pET43.1a+. Gene expression of cpcB was analyzed by sodium

SUBMITTER: Shoja Z 

PROVIDER: S-EPMC4600200 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature