Construction of cellulose-utilizing Escherichia coli based on a secretable cellulase.

ABSTRACT:

Background

The microbial conversion of plant biomass into value added products is an attractive option to address the impacts of petroleum dependency. The Gram-negative bacterium Escherichia coli is commonly used as host for the industrial production of various chemical products with a variety of sugars as carbon sources. However, this strain neither produces endogenous cellulose degradation enzymes nor secrets heterologous cellulases for its poor secretory capacity. Thus, a cellulolytic E. coli strain capable of growth on plant biomass would be the first step towards producing chemicals and fuels. We previously identified the catalytic domain of a cellulase (Cel-CD) and its N-terminal sequence (N20) that can serve as carriers for the efficient extracellular production of target en

SUBMITTER: Gao D 

PROVIDER: S-EPMC4600292 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature