Molecular Cloning, Expression and Purification of Truncated hpd Fragment of Haemophilus influenzae in Escherichia coli.

ABSTRACT:

Background

Nontypeable Haemophilus influenzae (NTHi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. Protein D (PD) belongs to the minor outer-membrane proteins of H. influenza. Moreover, it has been shown that this protein is one of the most potent vaccine candidates against the NTHi strain.

Objectives

In the present study, a new truncated form of PD was designed based on conserved areas, and recombinant truncated PD was expressed.

Materials and methods

Truncated PD was designed using bioinformatics tools, and a 345 bp fragment of the truncated hpd gene was amplified by polymerase chain reaction (PCR) from H. influenzae and subsequently cloned into the prokaryotic expression vect

SUBMITTER: Behrouzi A 

PROVIDER: S-EPMC4600343 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature