Dilution of the cell cycle inhibitor Whi5 controls budding-yeast cell size.

ABSTRACT: Cell size fundamentally affects all biosynthetic processes by determining the scale of organelles and influencing surface transport. Although extensive studies have identified many mutations affecting cell size, the molecular mechanisms underlying size control have remained elusive. In the budding yeast Saccharomyces cerevisiae, size control occurs in G1 phase before Start, the point of irreversible commitment to cell division. It was previously thought that activity of the G1 cyclin Cln3 increased with cell size to trigger Start by initiating the inhibition of the transcriptional inhibitor Whi5 (refs 6-8). Here we show that although Cln3 concentration does modulate the rate at which cells pass Start, its synthesis increases in proportion to cell size so that its total concentration is nea

SUBMITTER: Schmoller KM 

PROVIDER: S-EPMC4600446 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature