Comparison of cell stabilizing blood collection tubes for circulating plasma tumor DNA.

ABSTRACT:

Objectives

Circulating plasma DNA is being increasingly used for biomedical and clinical research as a substrate for genetic testing. However, cell lysis can occur hours after venipuncture when using standard tubes for blood collection, leading to an increase in contaminating cellular DNA that may hinder analysis of circulating plasma DNA. Cell stabilization agents can prevent cellular lysis for several days, reducing the need for immediate plasma preparation after venipuncture, thereby facilitating the ease of blood collection and sample preparation for clinical research. However, the majority of cell stabilizing reagents have not been formally tested for their ability to preserve circulating plasma tumor DNA.

Design & methods

In this study, we compared the properties of tw

SUBMITTER: Toro PV 

PROVIDER: S-EPMC4600652 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature