ABC Transporter CslAB, a Stabilizer of ComCDE Signal in Streptococcus mutans.

ABSTRACT:

Background

In Streptococcus mutans, ComCDE, a peptide-induced two-component signal transduction system, forms a closed signal transduction, and even if difunctional ComE closes this signal at its headstream to avoid its infinite amplification, it is not enough for ComE to work in a concentration-dependent manner. CslAB has a chance to regulate ComCDE by controlling extracellular competence-stimulating peptide (CSP) concentration through its processing and secretion.

Objectives

To first confirm the binding properties of cslAB promoter (PcslAB) with ComE, then to uncover in vivo need of cslAB expression, and finally to unveil the role of CslAB.

Materials and methods

Electrophoretic mobility shift assay was used to confirm the binding properties of PcslAB with ComE. In v

SUBMITTER: Liu T 

PROVIDER: S-EPMC4601000 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature