The basic helix-loop-helix transcription factor, Mist1, induces maturation of mouse fetal hepatoblasts.

ABSTRACT: Hepatic stem/progenitor cells, hepatoblasts, have a high proliferative ability and can differentiate into mature hepatocytes and cholangiocytes. Therefore, these cells are considered to be useful for regenerative medicine and drug screening for liver diseases. However, it is problem that in vitro maturation of hepatoblasts is insufficient in the present culture system. In this study, a novel regulator to induce hepatic differentiation was identified and the molecular function of this factor was examined in embryonic day 13 hepatoblast culture with maturation factor, oncostatin M and extracellular matrices. Overexpression of the basic helix-loop-helix type transcription factor, Mist1, induced expression of mature hepatocytic markers such as carbamoyl-phosphate synthetase1 and several cytoch

SUBMITTER: Chikada H 

PROVIDER: S-EPMC4601036 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature