In-Depth Assessment of Within-Individual and Inter-Individual Variation in the B Cell Receptor Repertoire.

ABSTRACT: High-throughput sequencing of the B cell receptor (BCR) repertoire can provide rapid characterization of the B cell response in a wide variety of applications in health, after vaccination and in infectious, inflammatory and immune-driven disease, and is starting to yield clinical applications. However, the interpretation of repertoire data is compromised by a lack of studies to assess the intra and inter-individual variation in the BCR repertoire over time in healthy individuals. We applied a standardized isotype-specific BCR repertoire deep sequencing protocol to a single highly sampled participant, and then evaluated the method in 9 further participants to comprehensively describe such variation. We assessed total repertoire metrics of mutation, diversity, VJ gene usage and isotype subcl

SUBMITTER: Galson JD 

PROVIDER: S-EPMC4601265 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature