Expression Change of miR-214 and miR-135 during Muscle Differentiation.

ABSTRACT:

Objective

MicroRNAs (miRNAs) are a class of small non-coding RNAs that play pivotal roles in many biological processes such as regulating skeletal muscle development where alterations in miRNA expression are reported during myogenesis. In this study, we aimed to investigate the impact of predicted miRNAs and their target genes on the myoblast to myocyte differentiation process.

Materials and methods

This experimental study was conducted on the C2C12 cell line. Using a bioinformatics approach, miR-214 and miR-135 were selected according to their targets as potential factors in myoblast to myocyte differentiation induced by 3% horse serum. Immunocytochemistry (ICC) was undertaken to confirm the differentiation process and quantitative real-time polymerase chain reaction (PCR)

SUBMITTER: Honardoost M 

PROVIDER: S-EPMC4601866 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature