High-Level Expression, Purification and Characterization of A Recombinant Plasmodium vivax Apical Membrane Antigen 1: Implication for vivax Malaria Vaccine Development.

ABSTRACT:

Objective

The apical membrane antigen-1 (AMA-1) is considered as a promising candidate for development of a malaria vaccine against Plasmodium parasites. The correct conformation of this protein appears to be necessary for the stimulation of parasite-inhibitory responses, and these responses, in turn, seem to be antibody-mediated. Therefore, in the present investigation, we expressed the Plasmodium vivax AMA-1 (PvAMA-1) ectodomain in Escherichia coli (E. coli), purified it using standard procedures and characterized it to determine its biological activities for it to be used as a potential target for developing a protective and safe vivax malaria vaccine.

Materials and methods

In this experimental investigation, the ectodomain of PvAMA-1 antigen (GenBank accession no. JX6247

SUBMITTER: Salavatifar M 

PROVIDER: S-EPMC4601873 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature