Spatial variation of the colonic microbiota in patients with ulcerative colitis and control volunteers.

ABSTRACT:

Objectives

The relevance of spatial composition in the microbial changes associated with UC is unclear. We coupled luminal brush samples, mucosal biopsies and laser capture microdissection with deep sequencing of the gut microbiota to develop an integrated spatial assessment of the microbial community in controls and UC.

Design

A total of 98 samples were sequenced to a mean depth of 31,642 reads from nine individuals, four control volunteers undergoing routine colonoscopy and five patients undergoing surgical colectomy for medically-refractory UC. Samples were retrieved at four colorectal locations, incorporating the luminal microbiota, mucus gel layer and whole mucosal biopsies.

Results

Interpersonal variability accounted for approximately half of the total variance.

SUBMITTER: Lavelle A 

PROVIDER: S-EPMC4602252 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature