Label-free Quantitative Analysis of Changes in Broiler Liver Proteins under Heat Stress using SWATH-MS Technology.

ABSTRACT: High temperature is one of the key environmental stressors affecting broiler production efficiency and meat yield. Knowledge of broiler self-regulation mechanisms under heat stress is important for the modern scale of poultry breeding. In the present study, the SWATH strategy was employed to investigate the temporal response of the broiler liver to heat stress. A total of 4,271 proteins were identified and used to generate a reference library for SWATH analysis. During this analysis, 2,377 proteins were quantified, with a coefficient of variation ≤25% among technical and biological replicates. A total of 257 proteins showed differential expression between the control and heat stressed groups. Consistent results for 26 and 5 differential proteins were validated respectively by MRM and weste

SUBMITTER: Tang X 

PROVIDER: S-EPMC4602270 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature