Time-lapse contact microscopy of cell cultures based on non-coherent illumination.

ABSTRACT: Video microscopy offers outstanding capabilities to investigate the dynamics of biological and pathological mechanisms in optimal culture conditions. Contact imaging is one of the simplest imaging architectures to digitally record images of cells due to the absence of any objective between the sample and the image sensor. However, in the framework of in-line holography, other optical components, e.g., an optical filter or a pinhole, are placed underneath the light source in order to illuminate the cells with a coherent or quasi-coherent incident light. In this study, we demonstrate that contact imaging with an incident light of both limited temporal and spatial coherences can be achieved with sufficiently high quality for most applications in cell biology, including monitoring of cell sedi

SUBMITTER: Gabriel M 

PROVIDER: S-EPMC4602279 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature