Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS.

ABSTRACT: Cost-effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions. Improving the performance of GH cocktails depends on knowledge of many critical parameters, including individual enzyme stabilities, optimal reaction conditions, kinetics, and specificity of reaction. With this information, rate- and/or yield-limiting reactions can be potentially improved through substitution, synergistic complementation, or protein engineering. Given the wide range of substrates and methods used for GH characterization, it is difficult to compare results across a myriad of approaches to identify high performance and synergistic combinations of enzymes. Here, we describe a platform

SUBMITTER: Deng K 

PROVIDER: S-EPMC4603251 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature