Transcriptome profiling of wheat glumes in wild emmer, hulled landraces and modern cultivars.

ABSTRACT:

Background

Wheat domestication is considered as one of the most important events in the development of human civilization. Wheat spikelets have undergone significant changes during evolution under domestication, resulting in soft glumes and larger kernels that are released easily upon threshing. Our main goal was to explore changes in transcriptome expression in glumes that accompanied wheat evolution under domestication.

Methods

A total of six tetraploid wheat accessions were selected for transcriptome profiling based on their rachis brittleness and glumes toughness. RNA pools from glumes of the central spikelet at heading time were used to construct cDNA libraries for sequencing. The trimmed reads from each library were separately aligned to the reference sub-genomes A and

SUBMITTER: Zou H 

PROVIDER: S-EPMC4603339 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature