Mining microsatellite markers from public expressed sequence tags databases for the study of threatened plants.

ABSTRACT:

Background

Simple Sequence Repeats (SSRs) are widely used in population genetic studies but their classical development is costly and time-consuming. The ever-increasing available DNA datasets generated by high-throughput techniques offer an inexpensive alternative for SSRs discovery. Expressed Sequence Tags (ESTs) have been widely used as SSR source for plants of economic relevance but their application to non-model species is still modest.

Methods

Here, we explored the use of publicly available ESTs (GenBank at the National Center for Biotechnology Information-NCBI) for SSRs development in non-model plants, focusing on genera listed by the International Union for the Conservation of Nature (IUCN). We also search two model genera with fully annotated genomes for EST-SSRs, A

SUBMITTER: Lopez L 

PROVIDER: S-EPMC4603344 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature