Genome-wide discovery and development of polymorphic microsatellites from Leishmania panamensis parasites circulating in central Panama.

ABSTRACT:

Background

The parasite Leishmania panamensis is the main cause of leishmaniasis in Panama. The disease is largely uncontrolled, with a rising incidence and no appropriate control measures. While microsatellites are considered some of the best genetic markers to study population genetics and molecular epidemiology in these and other parasites, none has been developed for L. panamensis.

Findings

Here we have developed and tested a new panel of microsatellites for this species, based on high-throughput genome-wide screening. The new set of microsatellites is composed of seventeen loci, mainly spanning trinucleotide or longer motifs. We have evaluated the sensitivity and specificity of the panel based on a sample of 27 isolates obtained from cutaneous leishmaniasis patients fro

SUBMITTER: Restrepo CM 

PROVIDER: S-EPMC4603350 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature