Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.

ABSTRACT: Regulated degradation of proteins by the proteasome is often critical to their function in dynamic cellular pathways. The molecular clock underlying mammalian circadian rhythms relies on the rhythmic expression and degradation of its core components. However, because the tools available for identifying the mechanisms underlying the degradation of a specific protein are limited, the mechanisms regulating clock protein degradation are only beginning to be elucidated. Here we describe a cell-based functional screening approach designed to quickly identify the ubiquitin E3 ligases that induce the degradation of potentially any protein of interest. We screened the nuclear hormone receptor RevErbα (Nr1d1), a key constituent of the mammalian circadian clock, for E3 ligases that regulate its stabi

SUBMITTER: DeBruyne JP 

PROVIDER: S-EPMC4603519 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature