Aberrant expression and localization of deoxyribonucleic acid methyltransferase 3B in endometriotic stromal cells.

ABSTRACT:

Objective

To define the expression and function of DNA methyltransferases (DNMTs) in response to decidualizing stimuli in endometriotic cells compared with healthy endometrial stroma.

Design

Basic science.

Setting

University research center.

Patient(s)

Premenopausal women with or without endometriosis.

Intervention(s)

Primary cultures of stromal cells from healthy endometrium (E-IUM) or endometriomas (E-OSIS) were subjected to in vitro decidualization (IVD) using 1 μM medroxyprogesterone acetate, 35 nM 17β-estradiol, and 0.05 mM 8-Br-cAMP.

Main outcome measure(s)

Expression of DNMT1, DNMT3A, and DNMT3B in E-IUM and E-OSIS were assessed by quantitative real-time polymerase chain reaction and immunoblotting. Recruitment of DNMT3B to the promoters of

SUBMITTER: Dyson MT 

PROVIDER: S-EPMC4603532 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature