Genetic characterization and improved genotyping of the dysferlin-deficient mouse strain Dysf (tm1Kcam).

ABSTRACT:

Background

Mouse models of dysferlinopathies are valuable tools with which to investigate the pathomechanisms underlying these diseases and to test novel therapeutic strategies. One such mouse model is the Dysf (tm1Kcam) strain, which was generated using a targeting vector to replace a 12-kb region of the dysferlin gene and which features a progressive muscular dystrophy. A prerequisite for successful animal studies using genetic mouse models is an accurate genotyping protocol. Unfortunately, the lack of robustness of currently available genotyping protocols for the Dysf (tm1Kcam) mouse has prevented efficient colony management. Initial attempts to improve the genotyping protocol based on the published genomic structure failed. These difficulties led us to analyze the targeted locu

SUBMITTER: Wiktorowicz T 

PROVIDER: S-EPMC4603641 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature