Rapid evolutionary divergence of Gossypium barbadense and G. hirsutum mitochondrial genomes.

ABSTRACT:

Background

The mitochondrial genome from upland cotton, G. hirsutum, was previously sequenced. To elucidate the evolution of mitochondrial genomic diversity within a single genus, we sequenced the mitochondrial genome from Sea Island cotton (Gossypium barbadense L.).

Methods

Mitochondrial DNA from week-old etiolated seedlings was extracted from isolated organelles using discontinuous sucrose density gradient method. Mitochondrial genome was sequenced with Solexa using paired-end, 90 bp read. The clean reads were assembled into contigs using ABySS and finished via additional fosmid and BAC sequencing. Finally, the genome was annotated and analyzed using different softwares.

Results

The G. barbadense (Sea Island cotton) mitochondrial genome was fully sequenced (677,434-

SUBMITTER: Tang M 

PROVIDER: S-EPMC4603758 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature