Identification of miRNAs involved in fruit ripening in Cavendish bananas by deep sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) are a family of non-coding small RNAs that play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are closely related to the ripening process in model plants. However, the miRNAs that are closely involved in the banana fruit ripening process remain unknown.

Methods

Here, we investigated the miRNA populations from banana fruits in response to ethylene or 1-MCP treatment using a deep sequencing approach and bioinformatics analysis combined with quantitative RT-PCR validation.

Results

A total of 125 known miRNAs and 26 novel miRNAs were identified from three libraries. MiRNA profiling of bananas in response to ethylene treatment compared with 1-MCP treatment showed differential expressio

SUBMITTER: Bi F 

PROVIDER: S-EPMC4603801 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature