Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315.

ABSTRACT:

Background

Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as opportunistic pathogen in humans. Infections with B. cenocepacia are very difficult to treat due to their high intrinsic resistance to most antibiotics. Biofilm formation further adds to their antibiotic resistance. B. cenocepacia harbours a large, multi-replicon genome with a high GC-content, the reference genome of strain J2315 includes 7374 annotated genes. This study aims to annotate transcription start sites and identify novel transcripts on a whole genome scale.

Methods

RNA extracted from B. cenocepacia J2315 biofilms was analysed by differential RNA-sequencing and the resulting dataset compared to data derived from conventional, global RNA-sequencing. Tra

SUBMITTER: Sass AM 

PROVIDER: S-EPMC4603805 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature