Establishment of monoclonal HCC cell lines with organ site-specific tropisms.

ABSTRACT:

Background

Organ site-specific metastasis is an ominous feature for most poor-prognostic hepatocellular carcinoma (HCC) patients. Cancer cell lines and animal models are indispensable for investigating the molecular mechanisms of organ specific tropism. However, till now, little is known about the drivers in HCC metastatic tropism, and also no effective way has been developed to block the process of tropistic metastasis.

Methods

In this study, we established several monoclonal HCC cell lines from HCCLM3-RFP together with their xenograft models, and then analyzed their metastatic potentials and tropisms using in-vitro and in-vivo assays, and finally elucidated the driving forces of HCC tropistic metastases.

Results

Six monoclonal cell lines with different organ site-sp

SUBMITTER: Wan J 

PROVIDER: S-EPMC4603809 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature