Enhanced direct fermentation of cassava to butanol by Clostridium species strain BOH3 in cofactor-mediated medium.

ABSTRACT:

Background

The main challenge of cassava-based biobutanol production is to enhance the simultaneous saccharification and fermentation with high hyperamylolytic activity and butanol yield. Manipulation of cofactor [e.g., Ca(2+) and NAD/(P)H] levels as a potential tool to modulate carbon flux plays a key role in the cassava hydrolysis capacity and butanol productivity. Here, we aimed to develop a technology for enhancing butanol production with simultaneous hydrolysis of cassava (a typical model as a non-cereal starchy material) using a cofactor-dependent modulation method to maximize the production efficacy of biobutanol by Clostridium sp. stain BOH3.

Results

Supplementing CaCO3 to the medium containing cassava significantly promotes activities of α-amylase responsible for ca

SUBMITTER: Li T 

PROVIDER: S-EPMC4603972 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature