Dissociation of skeletal muscle for flow cytometric characterization of immune cells in macaques.

ABSTRACT: The majority of vaccines and several treatments are administered by intramuscular injection. The aim is to engage and activate immune cells, although they are rare in normal skeletal muscle. The phenotype and function of resident as well as infiltrating immune cells in the muscle after injection are largely unknown. While methods for obtaining and characterizing murine muscle cell suspensions have been reported, protocols for nonhuman primates (NHPs) have not been well defined. NHPs comprise important in vivo models for studies of immune cell function due to their high degree of resemblance with humans. In this study, we developed and systematically compared methods to collect vaccine-injected muscle tissue to be processed into single cell suspensions for flow cytometric characterization o

SUBMITTER: Liang F 

PROVIDER: S-EPMC4604051 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature