Identification of Hippophae species (Shaji) through DNA barcodes.

ABSTRACT:

Background

The morphological identification of different Hippophae species (Shaji) was difficult. This study aims to discriminate between medicinal and non-medicinal Hippophae species by DNA barcodes, the ITS2, psbA-trnH, and a combination of ITS2 and psbA-trnH (ITS2 + psbA-trnH).

Methods

DNA was extracted from the dried fruit samples. Primer pairs ITS2F/3R for ITS2 and psbAF/trnHR for psbA-trnH were used for PCR amplification. The purified PCR products were bidirectionally sequenced. Genetic distances were calculated according to the Kimura 2 parameter model and phylogenetic tree was constructed based on neighbor-joining (NJ) method, barcoding gap was also analyzed to assess identification efficiency.

Results

Amplification and sequencing efficiencies for both ITS2 an

SUBMITTER: Liu Y 

PROVIDER: S-EPMC4604095 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature