On-chip Extraction of Intracellular Molecules in White Blood Cells from Whole Blood.

ABSTRACT: The extraction of virological markers in white blood cells (WBCs) from whole blood--without reagents, electricity, or instruments--is the most important first step for diagnostic testing of infectious diseases in resource-limited settings. Here we develop an integrated microfluidic chip that continuously separates WBCs from whole blood and mechanically ruptures them to extract intracellular proteins and nucleic acids for diagnostic purposes. The integrated chip is assembled with a device that separates WBCs by using differences in blood cell size and a mechanical cell lysis chip with ultra-sharp nanoblade arrays. We demonstrate the performance of the integrated device by quantitatively analyzing the levels of extracted intracellular proteins and genomic DNAs. Our results show that compared

SUBMITTER: Choi J 

PROVIDER: S-EPMC4604558 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature