High-throughput prediction of RNA, DNA and protein binding regions mediated by intrinsic disorder.

ABSTRACT: Intrinsically disordered proteins and regions (IDPs and IDRs) lack stable 3D structure under physiological conditions in-vitro, are common in eukaryotes, and facilitate interactions with RNA, DNA and proteins. Current methods for prediction of IDPs and IDRs do not provide insights into their functions, except for a handful of methods that address predictions of protein-binding regions. We report first-of-its-kind computational method DisoRDPbind for high-throughput prediction of RNA, DNA and protein binding residues located in IDRs from protein sequences. DisoRDPbind is implemented using a runtime-efficient multi-layered design that utilizes information extracted from physiochemical properties of amino acids, sequence complexity, putative secondary structure and disorder and sequence align

SUBMITTER: Peng Z 

PROVIDER: S-EPMC4605291 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature